Determination of Protein Solubility in Oilseed Meals Using Coomassie Blue Dye Binding
نویسندگان
چکیده
منابع مشابه
The solubility of Coomassie blue protein-dye complexes.
Coomassie blue protein dye-binding assays are commonly used for quantitation of protein. They exploit the interaction between protein and Coomassie brilliant blue (CBB) G-250, the latter being solubilized in either ethanol/phosphoric acid (1) or perchloric acid (2). The Bradford assay (1) is the method of choice. Potential problems with these assays include non-linearity, a variable response to...
متن کاملTotal protein determination in urine: elimination of a differential response between the coomassie blue and pyrogallol red protein dye-binding assays.
BACKGROUND The total protein content of urine is a good index of renal function, but its determination is unreliable. Protein dye-binding assays are simple, but they characteristically lack a uniform response to different proteins. METHODS We investigated a differential response of the Sigma Microprotein Coomassie Brilliant Blue (CBB) and Pyrogallol Red-molybdate (PRM) protein dye-binding ass...
متن کاملDetermining total protein content of urine: centrifugation results in precipitation of the protein-coomassie brilliant blue dye complex.
The Coomassie Brilliant Blue (CBB) protein dye-binding assay (1) has been favorably assessed for determining the total protein content of urine (2-4), although discrepancies have been noted in its response to different proteins (5) and sample dilution (6). It is commonly accepted that the proteinCBB dye complex is soluble under assay conditions, but our studies reveal loss of color yield after ...
متن کاملBinding of bioactive coomassie brilliant blue G with protein: Insights from spectroscopic studies
The binding of CBB to BSA was investigated under imitated physiological conditions employing different optical spectroscopic techniques viz., fluorescence emission, UV-vis absorption and FTIR. Fluorescence quenching data obtained at different temperatures suggested the presence of dynamic type of quenching mechanism. The values of K and n for CBB-BSA system were calculated and found to be 4.20 ...
متن کاملDimethylformamide interferes with Coomassie dye staining of proteins on blue native gel electrophoresis.
Blue native gel electrophoresis (BN-PAGE) is used extensively for characterization of mitochondrial respiratory complexes and uses the binding of Coomassie brilliant blue G-250 to visualize proteins. Oxidative modification of sulfhydryl groups of such proteins can be evaluated by labeling with iodoacetamide conjugated to biotin (BIAM) and detected with streptavidin peroxidase on Western blots f...
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ژورنال
عنوان ژورنال: Poultry Science
سال: 1993
ISSN: 0032-5791
DOI: 10.3382/ps.0721925